Journal: International Journal of Nanomedicine

Article Title: Periodontal Ligament Stem Cell Exosomes Key to Regulate Periodontal Regeneration by miR-31-5p in Mice Model

doi: 10.2147/IJN.S409664

Figure Lengend Snippet: Characterization of PDLSCs. ( A ) Schematic representation of PDLSCs culture. ( B ) Morphology of PDLSCs. Scale bar: 10 μm. ( C ) Alizarin red staining was used to show mineralized bone matrix formation after two weeks of osteogenesis induction. Scale bar: 100 μm. ( D ) Oil red staining was used to show lipid droplet formation after two weeks of lipid induction. Scale bar: 10 μm. ( E ) Immunofluorescence staining showed that the specific antigen STRO-1 was expressed on PDLSCs. Blue represents the nucleus stained with DAPI and red represents the cytoplasm stained with the STRO-1 antibody. Scale bar: 10 μm. ( F ) PDLSCs markers were detected by flow cytometry, positive expression of CD44, CD73, CD90 and negative expression of CD34 and CD45.

Article Snippet: Cells were immunolabeled for 4 hours with STRO-1 antibody (R&D Systems, Minneapolis, MN, USA) (1:1000) when they reached 80% confluence.

Techniques: Staining, Immunofluorescence, Flow Cytometry, Expressing

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Figure Lengend Snippet: The identification of the PDLSCs: the cells formed adherent clonogenic cell clusters of fibroblast-like cells (A), positive for STRO-1 (B) and vimention (C), while negative for cytokeratin (D) Multiple nodules were observed in the osteogenic medium after stained with alizarin red (E). Cells in the osteogenic medium present a greater ALP activity (F).

Article Snippet: Collect the cells on the third passage (2 × 10 7 ) in 2 mL of culture medium were incubated with monoclonal mouse anti-human STRO-1 (Abcam, USA) at a dilution of 1:100 (Serotec) for 30 min at 4°C.

Techniques: Staining, Activity Assay